Megadosages of vitamin C can reduce synthesis of acetylcholine

Megadosages of vitamin C can result in muscle pains and failures to synthesize cartilage where such difficulties do not remit unless dosages of vitamin C in more than around RDA dosages is stopped and uridine is supplemented. Megadosages of vitamin C decrease synthesis of uridine which leads to decreases in uridine triphosphate (UTP) which leads to decreases in UDP-glucose which leads to muscle pains and failures to synthesize cartilage. Low levels of uracil which is synthesized from uridine can lead to peripheral neuropathy due to failures to synthesize RNA. See this post.

Megadosages of vitamin C can also decrease synthesis of acetylcholine. Cytidine triphosphate (CTP) is synthesized from UTP by CTP synthase. CTP is used to synthesize CDP-choline by choline-phosphate cytidylyltransferase. 

CTP-choline is used to synthesize phosphatidylcholine. Choline in acetylcholine can derived from phosphatidylcholine. Decreases in levels of uridine due to megadosages of vitamin C could result is decreases of CTP, decreases in phosphatidylcholine and decreases in synthesis of acetylcholine.

CTP-choline is not supplemented. Low levels of CTP are due to low levels of uridine triphosphate. Megadosages of vitamin C are extremely toxic. More than around RDA dosages of vitamin C should not ever be supplemented.

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To increase levels of acetylcholine orotate from calcium orotate and magnesium orotate, inositol, N-acetylglucosamine and folic acid are supplemented. See this post.